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KMID : 1134820000290020312
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Journal of the Korean Society of Food Science and Nutrition
2000 Volume.29 No. 2 p.312 ~ p.315
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Purification of Macrophage Phagocytic Activity - Enhancing Component from Ethanol - Acetic Acid Extract of Korean Wheat
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Choi Myun
Kim Sun-Lyul
Kim Jong-Dai
Lee Sang-Young
Kim Hyun-Sook
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Abstract
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To purify the macrophage phagocytic activity-enhancing component, ethanol-acetic acid (100 : 1, EA) extract of Korean wheat (Gobun wheat) and imported one (Australian Standard White, ASW) were fractionated with ethylacetate : methanol : H2O (65 : 25 : 4, v/v/v), and identified by TLC and column chro- matography. At least five fractions were separated from the EA extract of the wheats but amounts of fraction B, C and D were more in Gobun wheat than in ASW. The effects of all fractions on phagocytic activity were tested in macrophage J774 cells. Among the fractions, only fraction b of Gobun wheat showed significant increase of phagocytic activity against yeast.
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KEYWORD
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Korean wheat, macrophage, phagocytic activity, purification
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